Murine Cell Line - Kras G12D, Lkb1 -/-, p53 -/-
Murine cell line Kras G12D/Lkb1 -/-, p53 -/- for studying Kras-driven cancers, such as non-small-cell lung cancer (NSCLC).
Technology No. WON02-09
LKR10 and LKR13 KrasG12D-mutant LUAD cells were transiently transfected with Keap1 CRISPR–Cas9 KO plasmid (sc-424513-KO-2) or Stk11/Lkb1 CRISPR–Cas9 KO plasmid (sc-423192). Lentiviral vectors encoding Cre (expressed from PGK promoter) and an sgRNA (expressed from human U6 promotor) target known or putative tumor suppressors that are recurrently mutated in LUAD (or pan-carcinoma), such as Trp53.