Murine Cell Line - Kras G12D, Lkb1 -/-, p53 -/-

Murine cell line Kras G12D/Lkb1 -/-, p53 -/- for studying Kras-driven cancers, such as non-small-cell lung cancer (NSCLC).
Technology No. WON02-09

LKR10 and LKR13 KrasG12D-mutant LUAD cells (on a 129/Sv genetic background, previously generated in the laboratory of T. Jacks) were transiently transfected with Keap1 CRISPR–Cas9 KO plasmid (sc-424513-KO-2) or Stk11/Lkb1 CRISPR–Cas9 KO plasmid (sc-423192). Lentiviral vectors encoding Cre (expressed from PGK promoter) and an sgRNA (expressed from human U6 promotor) target known or putative tumor suppressors that are recurrently mutated in LUAD (or pan-carcinoa), such as Trp53.

  • expand_more mode_edit Authors (1)
    Kwok-Kin Wong, MD, PhD
  • expand_more cloud_download Supporting documents (1)
    Product brochure
    Murine Cell Line - Kras G12D, Lkb1 -/-, p53 -/-.pdf
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