Casz1 floxed mice

Researchers have developed Casz1 floxed mice to investigate the role of the  Casz1 in modulating plasma lipoproteins and its impact on atherosclerosis. Mechanistic studies have identified Znf101/Zfp961 and Casz1 as transcription factors that enhance and repress apoB and apoA1, respectively. In previous experimental setups, female C57BL/6J mice were divided into groups and intravenously transduced with viruses expressing shRNA targeting Casz1 and Zfp961, and then fed a Western diet to induce hyperlipidemia.  This model provides a powerful tool for understanding the molecular mechanisms underlying lipid regulation and cardiovascular disease.