C57BL/6 (Rosa-rtTA shTet1)
These reversible RNAi transgenic mice can be used to model knockdown and restoration of endogeous Tet1 in hematopoietic cells for TET1 mutant diseases.
Technology No. AIF01-17
These are Doxycycline-inducible transgenic mice expressing shRNAs that target the Tet1 gene. To model reversible Tet1 knockdown, researchers created transgenic mice that express a Tet1-specific shRNA regulated by doxycycline (Dox) from the Col1a1 locus. These mice were bred with two types of transactivator mice: Vav-tTA (VTA) mice, which have a pan-hematopoietic, Dox-off tTA transactivator, and ROSA26-M2rtTA (RTA) mice, which have a ubiquitously expressed, Dox-on M2rtTA transactivator. The resulting progeny exhibit Dox-regulated Tet1 gene knockdown or restoration.